![]() This assay is designed to select and sequence polyadenylated transcripts. Our Core Facility has also used the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), for the initial amplification step for samples with low amounts of RNA (< 500 picograms of total RNA). However it is recommended to use at least 2 nanograms of total RNA per sample.Īssays from Takara (previously Clontech) and Illumina The minimum amount of RNA required is 500 picograms. To use this assay the samples should have concentrations greater than 100 picograms / microliter. I would recommend using this service, if the total quantity of your RNA per sample is less than 300 nanograms, and/or if your lab is interested in sequencing both polyadenylated and non-polyadenylated transcripts. The total cost per sample will include the cost for the amplification, library preparation, and sequencing. The Illumina protocol, the Nextera XT DNA Library Preparation Kit, is then used to create DNA library templates from the double stranded cDNA. Specifically the Tecan protocol, the Ovation RNA-Seq System v2, amplifies RNA samples between 500 picograms -10 nanograms, to create 2 -4 micrograms of double stranded cDNA. For most projects our facility uses RNA –Seq protocols from Tecan Genomics and Illumina. The Single Cell Genomics Core Facility provides a RNA-Seq service, for investigators interested in using Next Generation Sequencing technology, for samples with small quantities of total RNA. Assays from Tecan Genomics (previously NuGEN Technologies) and Illumina
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |